ADVANCED EQUINE INSIGHT
REPORTS GENOMIC ANALYSIS V4.1
54
VITALITY
CLINICAL REVIEW
CLINICAL REPORT · QUARTERLY

Atlas's Microbiome Profile

Subject AE-7855 · Sampled September 19, 2024 · Generated September 22, 2024
Diversity Index
41· Low
System Stability
62%
Pathogen Status
Detected
Executive Summary

What changed this quarter

Atlas's gut microbiome remains in the optimal performance band with a vitality score of 54. Fiber fermenter populations stabilized at +52.4% versus baseline; pathogen screening returned a secure result. A minor watch flag was raised on butyrate-producing populations — see clinical findings below.

Microbiome Landscape

Population profile

qmSeq · Species-Level
Fiber Fermenters
18% of profile
Markedly depressed — consistent with reduced forage retention.
Proteobacteria
22% of profile
Elevated Enterobacteriaceae signal. Clinical correlation advised.
Lactic Acid Producers
6% of profile
Lactobacillus depleted. Hindgut acidity at risk.
Butyrate Producers
9% of profile
Faecalibacterium below clinical reference threshold.
Starch Digesters
14% of profile
Within range but trending high — review concentrate ration.
Uncharacterized
31% of profile
Elevated unknown share — sample diversity floor.
favorable
neutral
caution
concern
References for this landscape · 7 sources

Horse species symposium: The microbiome of the horse hindgut: history and current knowledge

Julliand V, Grimm P · Journal of Animal Science · 2016

Review of hindgut microbial ecology, fiber-fermentation pathways, and the role of Fibrobacter and Lachnospiraceae populations.

DOI 10.2527/jas.2015-0198Illustrative · pending scientific review

Comparison of the fecal microbiota of healthy horses and horses with colitis by high throughput sequencing of the V3–V5 region of the 16S rRNA gene

Costa MC, Arroyo LG, et al. · PLOS ONE · 2012

Foundational study establishing the equine fecal microbiome baseline and characterizing dysbiosis signatures in clinical colitis.

DOI 10.1371/journal.pone.0041484Illustrative · pending scientific review

Comparison of the fecal microbiota in horses with equine metabolic syndrome and metabolically normal controls

Elzinga SE, Weese JS, Adams AA · Journal of Equine Veterinary Science · 2016

Identifies dysbiosis signatures specific to equine metabolic syndrome — informs caution flags on Proteobacteria elevations.

DOI 10.1016/j.jevs.2016.07.004Illustrative · pending scientific review

Characterization of the fecal bacteria communities of forage-fed horses by pyrosequencing of 16S rRNA V4 gene amplicons

Shepherd ML, Swecker WS, et al. · FEMS Microbiology Letters · 2012

Pyrosequencing characterization of forage-fed equine microbiota; supports fiber-fermenter reference ranges.

DOI 10.1111/j.1574-6968.2011.02411.xIllustrative · pending scientific review

Characterisation of the fecal microbiota of healthy adult horses

Stewart HL, Pitta D, et al. · Equine Veterinary Journal · 2018

Cohort study characterizing the fecal microbiota of healthy adult horses across stable management conditions; defines population reference envelopes.

DOI 10.1111/evj.12947Illustrative · pending scientific review

Influence of short-term dietary starch inclusion on the equine cecal microbiome

Warzecha CM, Coverdale JA, et al. · Journal of Animal Science · 2017

Shows acute starch challenge depresses fiber fermenters and elevates lactic-acid producers; supports starch-management recommendations.

DOI 10.2527/jas2017.1754Illustrative · pending scientific review

Bacterial diversity within the equine large intestine as revealed by molecular analysis of cloned 16S rRNA genes

Daly K, Stewart CS, et al. · FEMS Microbiology Ecology · 2001

Pioneering 16S analysis of equine large-intestinal bacterial diversity; cited for hindgut bacterial taxonomy.

DOI 10.1111/j.1574-6941.2001.tb00808.xIllustrative · pending scientific review
Bacteria Levels

Reference-range comparison

Fibrobacteres
Fibrobacter succinogenes
Bacteroidetes
12%7.1%22%
Streptococcus
Streptococcus equi (subsp.)
Firmicutes
0%6.8%4%
Proteobacteria
Enterobacteriaceae
Proteobacteria
0%9.2%4%
Lactobacillus
Lactobacillus spp.
Firmicutes
5%2.4%10%
References for these ranges · 8 sources

Horse species symposium: The microbiome of the horse hindgut: history and current knowledge

Julliand V, Grimm P · Journal of Animal Science · 2016

Review of hindgut microbial ecology, fiber-fermentation pathways, and the role of Fibrobacter and Lachnospiraceae populations.

DOI 10.2527/jas.2015-0198Illustrative · pending scientific review

Characterisation of the fecal microbiota of healthy adult horses

Stewart HL, Pitta D, et al. · Equine Veterinary Journal · 2018

Cohort study characterizing the fecal microbiota of healthy adult horses across stable management conditions; defines population reference envelopes.

DOI 10.1111/evj.12947Illustrative · pending scientific review

Strain dependent presence of Streptococcus equi subsp. equi in equine respiratory and digestive tracts

Blackmore TM, Dugdale A, et al. · Veterinary Microbiology · 2013

Documents S. equi subsp. presence in equine GI tracts; informs pathogen-screening thresholds.

DOI 10.1016/j.vetmic.2013.04.029Illustrative · pending scientific review

Pyrosequencing of 16S rRNA genes in fecal samples reveals high diversity of hindgut microflora in horses and potential links to chronic laminitis

Steelman SM, Chowdhary BP, et al. · BMC Veterinary Research · 2012

Links hindgut microbial signatures to chronic laminitis risk; basis for opportunistic-pathogen monitoring.

DOI 10.1186/1746-6148-8-231Illustrative · pending scientific review

Comparison of the fecal microbiota of healthy horses and horses with colitis by high throughput sequencing of the V3–V5 region of the 16S rRNA gene

Costa MC, Arroyo LG, et al. · PLOS ONE · 2012

Foundational study establishing the equine fecal microbiome baseline and characterizing dysbiosis signatures in clinical colitis.

DOI 10.1371/journal.pone.0041484Illustrative · pending scientific review

Comparison of the fecal microbiota in horses with equine metabolic syndrome and metabolically normal controls

Elzinga SE, Weese JS, Adams AA · Journal of Equine Veterinary Science · 2016

Identifies dysbiosis signatures specific to equine metabolic syndrome — informs caution flags on Proteobacteria elevations.

DOI 10.1016/j.jevs.2016.07.004Illustrative · pending scientific review

Characterization of the fecal bacteria communities of forage-fed horses by pyrosequencing of 16S rRNA V4 gene amplicons

Shepherd ML, Swecker WS, et al. · FEMS Microbiology Letters · 2012

Pyrosequencing characterization of forage-fed equine microbiota; supports fiber-fermenter reference ranges.

DOI 10.1111/j.1574-6968.2011.02411.xIllustrative · pending scientific review

Short-chain fructo-oligosaccharide supplementation effects on intestinal microflora and immune response of horses

Respondek F, Goachet AG, Julliand V · Journal of Animal Science · 2008

Evaluates prebiotic supplementation effects on equine gut microbial communities; cited for supplement-protocol rationale.

DOI 10.2527/jas.2007-0497Illustrative · pending scientific review
CRITICALF-IMMUNO

Critical Immunology Findings

Streptococcus equi subsp. detected at 6.8% relative abundance with concurrent Enterobacteriaceae bloom. Pattern consistent with active enteric inflammation; veterinarian review required within 48 hours.

  • S. equi subsp. abundance 6.8% (>4% threshold)
  • Enterobacteriaceae 9.2% (>4% threshold)
  • Diversity score 41 (low band)
Evidence · 3 sources

Strain dependent presence of Streptococcus equi subsp. equi in equine respiratory and digestive tracts

Blackmore TM, Dugdale A, et al. · Veterinary Microbiology · 2013

Documents S. equi subsp. presence in equine GI tracts; informs pathogen-screening thresholds.

DOI 10.1016/j.vetmic.2013.04.029Illustrative · pending scientific review

Comparison of the fecal microbiota of healthy horses and horses with colitis by high throughput sequencing of the V3–V5 region of the 16S rRNA gene

Costa MC, Arroyo LG, et al. · PLOS ONE · 2012

Foundational study establishing the equine fecal microbiome baseline and characterizing dysbiosis signatures in clinical colitis.

DOI 10.1371/journal.pone.0041484Illustrative · pending scientific review

Changes in the faecal microbiota of mares precede the development of post partum colic

Weese JS, Holcombe SJ, et al. · Equine Veterinary Journal · 2015

Demonstrates that shifts in fecal microbiota precede clinical colic — supports preemptive monitoring rationale.

DOI 10.1111/evj.12361Illustrative · pending scientific review
CAUTIONF-FIBER

Fibrobacter Population Depression

F. succinogenes at 7.1% — below the 12% reference floor. Reduced fiber fermentation capacity may compound pathogen load.

  • F. succinogenes 7.1% (target 12–22%)
  • Stability 62%
Evidence · 2 sources

Horse species symposium: The microbiome of the horse hindgut: history and current knowledge

Julliand V, Grimm P · Journal of Animal Science · 2016

Review of hindgut microbial ecology, fiber-fermentation pathways, and the role of Fibrobacter and Lachnospiraceae populations.

DOI 10.2527/jas.2015-0198Illustrative · pending scientific review

Influence of short-term dietary starch inclusion on the equine cecal microbiome

Warzecha CM, Coverdale JA, et al. · Journal of Animal Science · 2017

Shows acute starch challenge depresses fiber fermenters and elevates lactic-acid producers; supports starch-management recommendations.

DOI 10.2527/jas2017.1754Illustrative · pending scientific review
Recommendations

Actionable clinical guidance

Veterinary Review — 48 Hour Window

veterinary

Pathogen signal threshold exceeded. Initiate clinical review with Dr. Aris Thorne or attending vet.

Schedule consult; confirm culture; consider targeted PCR follow-up.
Duration · 2 days
Evidence · 2 sources

Strain dependent presence of Streptococcus equi subsp. equi in equine respiratory and digestive tracts

Blackmore TM, Dugdale A, et al. · Veterinary Microbiology · 2013

Documents S. equi subsp. presence in equine GI tracts; informs pathogen-screening thresholds.

DOI 10.1016/j.vetmic.2013.04.029Illustrative · pending scientific review

Changes in the faecal microbiota of mares precede the development of post partum colic

Weese JS, Holcombe SJ, et al. · Equine Veterinary Journal · 2015

Demonstrates that shifts in fecal microbiota precede clinical colic — supports preemptive monitoring rationale.

DOI 10.1111/evj.12361Illustrative · pending scientific review

Quarantine Protocol

monitoring

Standard biosecurity precaution while awaiting clinical confirmation.

Isolate from communal water; dedicated equipment; daily temperature log.
Duration · 14 days
Evidence · 1 source

Strain dependent presence of Streptococcus equi subsp. equi in equine respiratory and digestive tracts

Blackmore TM, Dugdale A, et al. · Veterinary Microbiology · 2013

Documents S. equi subsp. presence in equine GI tracts; informs pathogen-screening thresholds.

DOI 10.1016/j.vetmic.2013.04.029Illustrative · pending scientific review

Forage Restoration

nutrition

Restore F. succinogenes substrate availability post-clearance.

Long-stem grass hay free-choice; eliminate concentrate for 7 days; reintroduce gradually.
Duration · 30 days
Evidence · 3 sources

Horse species symposium: The microbiome of the horse hindgut: history and current knowledge

Julliand V, Grimm P · Journal of Animal Science · 2016

Review of hindgut microbial ecology, fiber-fermentation pathways, and the role of Fibrobacter and Lachnospiraceae populations.

DOI 10.2527/jas.2015-0198Illustrative · pending scientific review

The effects of weaning methods on gut microbiota composition and horse physiology

Mach N, Foury A, et al. · Frontiers in Physiology · 2017

Examines diet-shift impacts on equine gut microbial composition; basis for transition-protocol guidance.

DOI 10.3389/fphys.2017.00535Illustrative · pending scientific review

Use of fecal microbial transplantation in horses

Arroyo LG, Rossi L, et al. · Journal of Veterinary Internal Medicine · 2017

Evaluates fecal microbial transplant as adjunct intervention in dysbiotic equine cases; informs restorative protocols.

DOI 10.1111/jvim.14773Illustrative · pending scientific review
Authorized Clinical Verification Required
All clinical interpretations must be reviewed by a licensed veterinarian before implementing protocol changes. CLIA #44D2148991 · Genomic Sciences Lab.
CLINICAL REVIEW