ADVANCED EQUINE INSIGHT
REPORTS GENOMIC ANALYSIS V4.1
68
VITALITY
CLINICAL REVIEW
CLINICAL REPORT · QUARTERLY

Juno's Microbiome Profile

Subject AE-7801 · Sampled September 12, 2024 · Generated September 18, 2024
Diversity Index
61· Moderate
System Stability
71%
Pathogen Status
Watch
Reviewed by Dr. Aris Thorne
Chief Researcher
Executive Summary

What changed this quarter

Juno's gut microbiome remains in the optimal performance band with a vitality score of 68. Fiber fermenter populations stabilized at +52.4% versus baseline; pathogen screening returned a secure result. A minor watch flag was raised on butyrate-producing populations — see clinical findings below.

Microbiome Landscape

Population profile

qmSeq · Species-Level
Fiber Fermenters
38% of profile
Structural carbohydrate processing performing within optimal envelope.
Lactic Acid Producers
12% of profile
Lactobacillus and Bifidobacterium populations supporting hindgut homeostasis.
Butyrate Producers
19% of profile
Lachnospiraceae stable; minor Faecalibacterium decrease noted vs. Q2.
Starch Digesters
8% of profile
Within reference range — monitor during competition season grain shifts.
Proteobacteria
4% of profile
Low and stable; no signal of dysbiosis.
Uncharacterized
19% of profile
Species below the species-level identification confidence threshold.
favorable
neutral
caution
concern
References for this landscape · 7 sources

Horse species symposium: The microbiome of the horse hindgut: history and current knowledge

Julliand V, Grimm P · Journal of Animal Science · 2016

Review of hindgut microbial ecology, fiber-fermentation pathways, and the role of Fibrobacter and Lachnospiraceae populations.

DOI 10.2527/jas.2015-0198Illustrative · pending scientific review

Characterization of the fecal bacteria communities of forage-fed horses by pyrosequencing of 16S rRNA V4 gene amplicons

Shepherd ML, Swecker WS, et al. · FEMS Microbiology Letters · 2012

Pyrosequencing characterization of forage-fed equine microbiota; supports fiber-fermenter reference ranges.

DOI 10.1111/j.1574-6968.2011.02411.xIllustrative · pending scientific review

Short-chain fructo-oligosaccharide supplementation effects on intestinal microflora and immune response of horses

Respondek F, Goachet AG, Julliand V · Journal of Animal Science · 2008

Evaluates prebiotic supplementation effects on equine gut microbial communities; cited for supplement-protocol rationale.

DOI 10.2527/jas.2007-0497Illustrative · pending scientific review

Characterisation of the fecal microbiota of healthy adult horses

Stewart HL, Pitta D, et al. · Equine Veterinary Journal · 2018

Cohort study characterizing the fecal microbiota of healthy adult horses across stable management conditions; defines population reference envelopes.

DOI 10.1111/evj.12947Illustrative · pending scientific review

Influence of short-term dietary starch inclusion on the equine cecal microbiome

Warzecha CM, Coverdale JA, et al. · Journal of Animal Science · 2017

Shows acute starch challenge depresses fiber fermenters and elevates lactic-acid producers; supports starch-management recommendations.

DOI 10.2527/jas2017.1754Illustrative · pending scientific review

Comparison of the fecal microbiota in horses with equine metabolic syndrome and metabolically normal controls

Elzinga SE, Weese JS, Adams AA · Journal of Equine Veterinary Science · 2016

Identifies dysbiosis signatures specific to equine metabolic syndrome — informs caution flags on Proteobacteria elevations.

DOI 10.1016/j.jevs.2016.07.004Illustrative · pending scientific review

Bacterial diversity within the equine large intestine as revealed by molecular analysis of cloned 16S rRNA genes

Daly K, Stewart CS, et al. · FEMS Microbiology Ecology · 2001

Pioneering 16S analysis of equine large-intestinal bacterial diversity; cited for hindgut bacterial taxonomy.

DOI 10.1111/j.1574-6941.2001.tb00808.xIllustrative · pending scientific review
Bacteria Levels

Reference-range comparison

Fibrobacteres
Fibrobacter succinogenes
Bacteroidetes
12%18.4%22%
Lactobacillus
Lactobacillus spp.
Firmicutes
5%7.2%10%
Lachnospiraceae
Lachnospiraceae family
Firmicutes
10%14.1%18%
Streptococcus
Streptococcus bovis
Firmicutes
0%1.1%4%
Proteobacteria
γ-Proteobacteria
Proteobacteria
0%2.3%4%
Bifidobacterium
Bifidobacterium spp.
Actinobacteria
2%3.4%6%
References for these ranges · 6 sources

Characterisation of the fecal microbiota of healthy adult horses

Stewart HL, Pitta D, et al. · Equine Veterinary Journal · 2018

Cohort study characterizing the fecal microbiota of healthy adult horses across stable management conditions; defines population reference envelopes.

DOI 10.1111/evj.12947Illustrative · pending scientific review

Horse species symposium: The microbiome of the horse hindgut: history and current knowledge

Julliand V, Grimm P · Journal of Animal Science · 2016

Review of hindgut microbial ecology, fiber-fermentation pathways, and the role of Fibrobacter and Lachnospiraceae populations.

DOI 10.2527/jas.2015-0198Illustrative · pending scientific review

Characterization of the fecal bacteria communities of forage-fed horses by pyrosequencing of 16S rRNA V4 gene amplicons

Shepherd ML, Swecker WS, et al. · FEMS Microbiology Letters · 2012

Pyrosequencing characterization of forage-fed equine microbiota; supports fiber-fermenter reference ranges.

DOI 10.1111/j.1574-6968.2011.02411.xIllustrative · pending scientific review

Pyrosequencing of 16S rRNA genes in fecal samples reveals high diversity of hindgut microflora in horses and potential links to chronic laminitis

Steelman SM, Chowdhary BP, et al. · BMC Veterinary Research · 2012

Links hindgut microbial signatures to chronic laminitis risk; basis for opportunistic-pathogen monitoring.

DOI 10.1186/1746-6148-8-231Illustrative · pending scientific review

Comparison of the fecal microbiota in horses with equine metabolic syndrome and metabolically normal controls

Elzinga SE, Weese JS, Adams AA · Journal of Equine Veterinary Science · 2016

Identifies dysbiosis signatures specific to equine metabolic syndrome — informs caution flags on Proteobacteria elevations.

DOI 10.1016/j.jevs.2016.07.004Illustrative · pending scientific review

Short-chain fructo-oligosaccharide supplementation effects on intestinal microflora and immune response of horses

Respondek F, Goachet AG, Julliand V · Journal of Animal Science · 2008

Evaluates prebiotic supplementation effects on equine gut microbial communities; cited for supplement-protocol rationale.

DOI 10.2527/jas.2007-0497Illustrative · pending scientific review
INFOF-FIBER

Fiber Utilization Optimized

Fibrobacteres succinogenes population stabilized at +52.4% vs. baseline. Hindgut fermentation efficiency estimated at 98.4% — peak performance band.

  • F. succinogenes 18.4% (target 12–22%)
  • Stability 94%
Evidence · 3 sources

Horse species symposium: The microbiome of the horse hindgut: history and current knowledge

Julliand V, Grimm P · Journal of Animal Science · 2016

Review of hindgut microbial ecology, fiber-fermentation pathways, and the role of Fibrobacter and Lachnospiraceae populations.

DOI 10.2527/jas.2015-0198Illustrative · pending scientific review

Characterisation of the fecal microbiota of healthy adult horses

Stewart HL, Pitta D, et al. · Equine Veterinary Journal · 2018

Cohort study characterizing the fecal microbiota of healthy adult horses across stable management conditions; defines population reference envelopes.

DOI 10.1111/evj.12947Illustrative · pending scientific review

Characterisation of the faecal metabolome and microbiome of Thoroughbred racehorses

Proudman CJ, Hunter JO, et al. · Equine Veterinary Journal · 2015

Integrated metabolome–microbiome characterization of performance Thoroughbreds, linking diet to short-chain fatty acid output.

DOI 10.1111/evj.12324Illustrative · pending scientific review
WATCHF-BUTYRATE

Butyrate Producer Trend — Watch

Faecalibacterium subset shows -7.1% over the past two quarters. Trajectory remains within reference range; recommend monitor at next quarterly cycle.

  • Faecalibacterium prausnitzii decreased 7.1%
  • Within reference range
Evidence · 2 sources

Characterisation of the fecal microbiota of healthy adult horses

Stewart HL, Pitta D, et al. · Equine Veterinary Journal · 2018

Cohort study characterizing the fecal microbiota of healthy adult horses across stable management conditions; defines population reference envelopes.

DOI 10.1111/evj.12947Illustrative · pending scientific review

Changes in the faecal microbiota of mares precede the development of post partum colic

Weese JS, Holcombe SJ, et al. · Equine Veterinary Journal · 2015

Demonstrates that shifts in fecal microbiota precede clinical colic — supports preemptive monitoring rationale.

DOI 10.1111/evj.12361Illustrative · pending scientific review
Recommendations

Actionable clinical guidance

Maintain Fiber Ratio

nutrition

Current Lachnospiraceae populations indicate hindgut homeostasis under existing forage profile.

Continue 70/30 mixed-stem hay with 15% increase in mid-bloom timothy through next cycle.
Duration · 90 days
Evidence · 2 sources

Horse species symposium: The microbiome of the horse hindgut: history and current knowledge

Julliand V, Grimm P · Journal of Animal Science · 2016

Review of hindgut microbial ecology, fiber-fermentation pathways, and the role of Fibrobacter and Lachnospiraceae populations.

DOI 10.2527/jas.2015-0198Illustrative · pending scientific review

The effects of weaning methods on gut microbiota composition and horse physiology

Mach N, Foury A, et al. · Frontiers in Physiology · 2017

Examines diet-shift impacts on equine gut microbial composition; basis for transition-protocol guidance.

DOI 10.3389/fphys.2017.00535Illustrative · pending scientific review

Butyrate Support Protocol

supplement

Pre-emptive support for Faecalibacterium populations approaching low band.

Sodium butyrate 4g/day for a 30-day cycle; reassess at next quarterly sample.
Duration · 30 days
Evidence · 2 sources

Short-chain fructo-oligosaccharide supplementation effects on intestinal microflora and immune response of horses

Respondek F, Goachet AG, Julliand V · Journal of Animal Science · 2008

Evaluates prebiotic supplementation effects on equine gut microbial communities; cited for supplement-protocol rationale.

DOI 10.2527/jas.2007-0497Illustrative · pending scientific review

Effect of short-term Saccharomyces cerevisiae supplementation on the fecal microbiota of horses

Tavenner MK, McDonnell SM, Biddle AS · Animal Microbiome · 2020

Demonstrates probiotic-supplementation effects on equine microbiota; basis for supplement-trial protocols.

DOI 10.1186/s42523-020-00046-4Illustrative · pending scientific review
Authorized Clinical Verification Required
All clinical interpretations must be reviewed by a licensed veterinarian before implementing protocol changes. CLIA #44D2148991 · Genomic Sciences Lab.
WATCH